Construct: shRNA TRCN0000120594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010785.1-535s1c1
DNA Barcode:
GATAGGCATCTTCGTAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mdm1 (17245)
Gene Description:
transformed mouse 3T3 cell double minute 1
Transcript:
RefSeq NM_010785.1 (NON-CURRENT)
Match location:
Position 535 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGGCATCTTCGTAAGAAA
Hairpin Sequence:
5'-CCGG-GATAGGCATCTTCGTAAGAAA-CTCGAG-TTTCTTACGAAGATGCCTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGGCATCTTCGTAAGAAACTCGAGTTTCTTACGAAGATGCCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGGCATCTTCGTAAGAAACTCGAGTTTCTTACGAAGATGCCTATC-3'

Other clones with same target sequence:

(none)