Construct: shRNA TRCN0000120622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009012.1-4594s1c1
DNA Barcode:
GCTGGCTGTTAGAGAAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad50 (19360)
Gene Description:
RAD50 double strand break repair protein
Transcript:
RefSeq NM_009012.1 (NON-CURRENT)
Match location:
Position 4594 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCTGTTAGAGAAGAGTT
Hairpin Sequence:
5'-CCGG-GCTGGCTGTTAGAGAAGAGTT-CTCGAG-AACTCTTCTCTAACAGCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCTGTTAGAGAAGAGTTCTCGAGAACTCTTCTCTAACAGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCTGTTAGAGAAGAGTTCTCGAGAACTCTTCTCTAACAGCCAGC-3'

Other clones with same target sequence:

(none)