Construct: shRNA TRCN0000120624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009012.1-990s1c1
DNA Barcode:
CCATTGAAGAATCGCCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad50 (19360)
Gene Description:
RAD50 double strand break repair protein
Transcript:
RefSeq NM_009012.1 (NON-CURRENT)
Match location:
Position 990 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGAAGAATCGCCTGAAA
Hairpin Sequence:
5'-CCGG-CCATTGAAGAATCGCCTGAAA-CTCGAG-TTTCAGGCGATTCTTCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGAAGAATCGCCTGAAACTCGAGTTTCAGGCGATTCTTCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGAAGAATCGCCTGAAACTCGAGTTTCAGGCGATTCTTCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000336380