Construct: shRNA TRCN0000120649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019480.3-367s1c1
DNA Barcode:
GCAACAGTGTTCTCGTTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ebag9 (55960)
Gene Description:
estrogen receptor-binding fragment-associated gene 9
Transcript:
RefSeq NM_019480.3 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAGTGTTCTCGTTCCTA
Hairpin Sequence:
5'-CCGG-GCAACAGTGTTCTCGTTCCTA-CTCGAG-TAGGAACGAGAACACTGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAGTGTTCTCGTTCCTACTCGAGTAGGAACGAGAACACTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAGTGTTCTCGTTCCTACTCGAGTAGGAACGAGAACACTGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000314440