Construct: shRNA TRCN0000120659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026008.1-622s1c1
DNA Barcode:
CCTCCTCTCATTGCTGAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sclt1 (67161)
Gene Description:
sodium channel and clathrin linker 1
Transcript:
RefSeq NM_026008.1 (NON-CURRENT)
Match location:
Position 622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTCTCATTGCTGAGTAT
Hairpin Sequence:
5'-CCGG-CCTCCTCTCATTGCTGAGTAT-CTCGAG-ATACTCAGCAATGAGAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTCTCATTGCTGAGTATCTCGAGATACTCAGCAATGAGAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTCTCATTGCTGAGTATCTCGAGATACTCAGCAATGAGAGGAGG-3'

Other clones with same target sequence:

(none)