Construct: shRNA TRCN0000120665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026917.4-242s1c1
DNA Barcode:
GCCATGTGGTTTATCCGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc3 (69035)
Gene Description:
zinc finger, DHHC domain containing 3
Transcript:
RefSeq NM_026917.4 (NON-CURRENT)
Match location:
Position 242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTGGTTTATCCGAGAT
Hairpin Sequence:
5'-CCGG-GCCATGTGGTTTATCCGAGAT-CTCGAG-ATCTCGGATAAACCACATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTGGTTTATCCGAGATCTCGAGATCTCGGATAAACCACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTGGTTTATCCGAGATCTCGAGATCTCGGATAAACCACATGGC-3'

Other clones with same target sequence:

  1. TRCN0000345216