Construct: shRNA TRCN0000120667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029620.1-1496s1c1
DNA Barcode:
CGCTATTTAATGCTGAGCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcolce2 (76477)
Gene Description:
procollagen C-endopeptidase enhancer 2
Transcript:
RefSeq NM_029620.1 (NON-CURRENT)
Match location:
Position 1496 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATTTAATGCTGAGCGTT
Hairpin Sequence:
5'-CCGG-CGCTATTTAATGCTGAGCGTT-CTCGAG-AACGCTCAGCATTAAATAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATTTAATGCTGAGCGTTCTCGAGAACGCTCAGCATTAAATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATTTAATGCTGAGCGTTCTCGAGAACGCTCAGCATTAAATAGCG-3'

Other clones with same target sequence:

  1. TRCN0000345357