Construct: shRNA TRCN0000120737
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_026531.2-1361s1c1
- DNA Barcode:
- CCACAGCATGAGGAACTGTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Aen (68048)
- Gene Description:
- apoptosis enhancing nuclease
- Transcript:
- RefSeq NM_026531.2 (NON-CURRENT)
- Match location:
- Position 1361 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000120737)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCACAGCATGAGGAACTGTAA
- Hairpin Sequence:
-
5'-CCGG-CCACAGCATGAGGAACTGTAA-CTCGAG-TTACAGTTCCTCATGCTGTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCACAGCATGAGGAACTGTAACTCGAGTTACAGTTCCTCATGCTGTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCACAGCATGAGGAACTGTAACTCGAGTTACAGTTCCTCATGCTGTGG-3'
Other clones with same target sequence:
(none)
