Construct: shRNA TRCN0000120741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026531.2-204s1c1
DNA Barcode:
CCTGAATACCAAGGATGTGGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aen (68048)
Gene Description:
apoptosis enhancing nuclease
Transcript:
RefSeq NM_026531.2 (NON-CURRENT)
Match location:
Position 204 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAATACCAAGGATGTGGT
Hairpin Sequence:
5'-CCGG-CCTGAATACCAAGGATGTGGT-CTCGAG-ACCACATCCTTGGTATTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAATACCAAGGATGTGGTCTCGAGACCACATCCTTGGTATTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAATACCAAGGATGTGGTCTCGAGACCACATCCTTGGTATTCAGG-3'

Other clones with same target sequence:

(none)