Construct: shRNA TRCN0000120895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178851.1-2220s1c1
DNA Barcode:
CCACGTCTTGAAGGACACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps39 (269338)
Gene Description:
VPS39 HOPS complex subunit
Transcript:
RefSeq NM_178851.1 (NON-CURRENT)
Match location:
Position 2220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGTCTTGAAGGACACAAA
Hairpin Sequence:
5'-CCGG-CCACGTCTTGAAGGACACAAA-CTCGAG-TTTGTGTCCTTCAAGACGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGTCTTGAAGGACACAAACTCGAGTTTGTGTCCTTCAAGACGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGTCTTGAAGGACACAAACTCGAGTTTGTGTCCTTCAAGACGTGG-3'

Other clones with same target sequence:

  1. TRCN0000345239