Construct: shRNA TRCN0000120936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021568.1-1170s1c1
DNA Barcode:
TGATCTTATAGGCTGCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcbp3 (59093)
Gene Description:
poly(rC) binding protein 3
Transcript:
RefSeq NM_021568.1 (NON-CURRENT)
Match location:
Position 1170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCTTATAGGCTGCATAAT
Hairpin Sequence:
5'-CCGG-TGATCTTATAGGCTGCATAAT-CTCGAG-ATTATGCAGCCTATAAGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCTTATAGGCTGCATAATCTCGAGATTATGCAGCCTATAAGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCTTATAGGCTGCATAATCTCGAGATTATGCAGCCTATAAGATCA-3'

Other clones with same target sequence:

  1. TRCN0000340517