Construct: shRNA TRCN0000120945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145529.1-1519s1c1
DNA Barcode:
CACCTCAATGAGGACAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cstf3 (228410)
Gene Description:
cleavage stimulation factor, 3' pre-RNA, subunit 3
Transcript:
RefSeq NM_145529.1 (NON-CURRENT)
Match location:
Position 1519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTCAATGAGGACAATAAT
Hairpin Sequence:
5'-CCGG-CACCTCAATGAGGACAATAAT-CTCGAG-ATTATTGTCCTCATTGAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTCAATGAGGACAATAATCTCGAGATTATTGTCCTCATTGAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTCAATGAGGACAATAATCTCGAGATTATTGTCCTCATTGAGGTG-3'

Other clones with same target sequence:

(none)