Construct: shRNA TRCN0000120956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_126554.5-2164s1c1
DNA Barcode:
GACCTCTACATAAAGGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Helz (78455)
Gene Description:
helicase with zinc finger domain
Transcript:
RefSeq XM_126554.5 (NON-CURRENT)
Match location:
Position 2164 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCTACATAAAGGATTAT
Hairpin Sequence:
5'-CCGG-GACCTCTACATAAAGGATTAT-CTCGAG-ATAATCCTTTATGTAGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCTACATAAAGGATTATCTCGAGATAATCCTTTATGTAGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCTACATAAAGGATTATCTCGAGATAATCCTTTATGTAGAGGTC-3'

Other clones with same target sequence:

(none)