Construct: shRNA TRCN0000120957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015792.1-3300s1c1
DNA Barcode:
GCATCTCTGAGGAGGAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo18 (50755)
Gene Description:
F-box protein 18
Transcript:
RefSeq NM_015792.1 (NON-CURRENT)
Match location:
Position 3300 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTCTGAGGAGGAAGATA
Hairpin Sequence:
5'-CCGG-GCATCTCTGAGGAGGAAGATA-CTCGAG-TATCTTCCTCCTCAGAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTCTGAGGAGGAAGATACTCGAGTATCTTCCTCCTCAGAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTCTGAGGAGGAAGATACTCGAGTATCTTCCTCCTCAGAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000353414