Construct: shRNA TRCN0000121037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027760.1-1537s1c1
DNA Barcode:
GCAAAGCCTTTGTTTGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf8 (71323)
Gene Description:
Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Transcript:
RefSeq NM_027760.1 (NON-CURRENT)
Match location:
Position 1537 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGCCTTTGTTTGTATTT
Hairpin Sequence:
5'-CCGG-GCAAAGCCTTTGTTTGTATTT-CTCGAG-AAATACAAACAAAGGCTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGCCTTTGTTTGTATTTCTCGAGAAATACAAACAAAGGCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGCCTTTGTTTGTATTTCTCGAGAAATACAAACAAAGGCTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000345565