Construct: shRNA TRCN0000121043
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145398.2-734s1c1
- DNA Barcode:
- CCAGTTTATGAGGATCTCTTA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Casd1 (213819)
- Gene Description:
- CAS1 domain containing 1
- Transcript:
- RefSeq NM_145398.2 (CURRENT)
- Match location:
- Position 734 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000121043)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCAGTTTATGAGGATCTCTTA
- Hairpin Sequence:
- 5'-CCGG-CCAGTTTATGAGGATCTCTTA-CTCGAG-TAAGAGATCCTCATAAACTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGTTTATGAGGATCTCTTACTCGAGTAAGAGATCCTCATAAACTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGTTTATGAGGATCTCTTACTCGAGTAAGAGATCCTCATAAACTGG-3'