Construct: shRNA TRCN0000121045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145398.2-2381s1c1
DNA Barcode:
GCGCAGGTCGTTATTCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Casd1 (213819)
Gene Description:
CAS1 domain containing 1
Transcript:
RefSeq NM_145398.2 (CURRENT)
Match location:
Position 2381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAGGTCGTTATTCCTAAA
Hairpin Sequence:
5'-CCGG-GCGCAGGTCGTTATTCCTAAA-CTCGAG-TTTAGGAATAACGACCTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAGGTCGTTATTCCTAAACTCGAGTTTAGGAATAACGACCTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAGGTCGTTATTCCTAAACTCGAGTTTAGGAATAACGACCTGCGC-3'

Other clones with same target sequence:

(none)