Construct: shRNA TRCN0000121137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001569.3-2873s1c1
DNA Barcode:
GCTGAAGTAGGAGGATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Gene Description:
interleukin 1 receptor associated kinase 1
Transcript:
RefSeq NM_001569.3 (NON-CURRENT)
Match location:
Position 2873 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGTAGGAGGATCATTT
Hairpin Sequence:
5'-CCGG-GCTGAAGTAGGAGGATCATTT-CTCGAG-AAATGATCCTCCTACTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGTAGGAGGATCATTTCTCGAGAAATGATCCTCCTACTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGTAGGAGGATCATTTCTCGAGAAATGATCCTCCTACTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000342719