Construct: shRNA TRCN0000121176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006182.2-1419s1c1
DNA Barcode:
CCATCAAGTGTCAATACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDR2 (4921)
Gene Description:
discoidin domain receptor tyrosine kinase 2
Transcript:
RefSeq NM_006182.2 (NON-CURRENT)
Match location:
Position 1419 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAAGTGTCAATACCATT
Hairpin Sequence:
5'-CCGG-CCATCAAGTGTCAATACCATT-CTCGAG-AATGGTATTGACACTTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAAGTGTCAATACCATTCTCGAGAATGGTATTGACACTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAAGTGTCAATACCATTCTCGAGAATGGTATTGACACTTGATGG-3'

Other clones with same target sequence:

(none)