Construct: shRNA TRCN0000121227
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005433.3-2684s1c1
- DNA Barcode:
- GCAAAGTAAGACCCAGCATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- YES1 (7525)
- Gene Description:
- YES proto-oncogene 1, Src family tyrosine kinase
- Transcript:
- RefSeq NM_005433.3 (NON-CURRENT)
- Match location:
- Position 2684 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000121227)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAAAGTAAGACCCAGCATTT
- Hairpin Sequence:
-
5'-CCGG-GCAAAGTAAGACCCAGCATTT-CTCGAG-AAATGCTGGGTCTTACTTTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAAGTAAGACCCAGCATTTCTCGAGAAATGCTGGGTCTTACTTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAAGTAAGACCCAGCATTTCTCGAGAAATGCTGGGTCTTACTTTGC-3'
Other clones with same target sequence:
(none)
