Construct: shRNA TRCN0000121230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005433.3-669s1c1
DNA Barcode:
GCAGATTCCATTCAGGCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YES1 (7525)
Gene Description:
YES proto-oncogene 1, Src family tyrosine kinase
Transcript:
RefSeq NM_005433.3 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTCCATTCAGGCAGAA
Hairpin Sequence:
5'-CCGG-GCAGATTCCATTCAGGCAGAA-CTCGAG-TTCTGCCTGAATGGAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTCCATTCAGGCAGAACTCGAGTTCTGCCTGAATGGAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTCCATTCAGGCAGAACTCGAGTTCTGCCTGAATGGAATCTGC-3'

Other clones with same target sequence:

(none)