Construct: shRNA TRCN0000121240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001569.3-124s1c1
DNA Barcode:
CCAGCACTTCTTGTACGAGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Gene Description:
interleukin 1 receptor associated kinase 1
Transcript:
RefSeq NM_001569.3 (NON-CURRENT)
Match location:
Position 124 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCACTTCTTGTACGAGGT
Hairpin Sequence:
5'-CCGG-CCAGCACTTCTTGTACGAGGT-CTCGAG-ACCTCGTACAAGAAGTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCACTTCTTGTACGAGGTCTCGAGACCTCGTACAAGAAGTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCACTTCTTGTACGAGGTCTCGAGACCTCGTACAAGAAGTGCTGG-3'

Other clones with same target sequence:

(none)