Construct: shRNA TRCN0000121253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002447.1-3140s1c1
DNA Barcode:
GCATCCTTCTCCGATAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MST1R (4486)
Gene Description:
macrophage stimulating 1 receptor
Transcript:
RefSeq NM_002447.1 (NON-CURRENT)
Match location:
Position 3140 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCTTCTCCGATAGTGAA
Hairpin Sequence:
5'-CCGG-GCATCCTTCTCCGATAGTGAA-CTCGAG-TTCACTATCGGAGAAGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCTTCTCCGATAGTGAACTCGAGTTCACTATCGGAGAAGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCTTCTCCGATAGTGAACTCGAGTTCACTATCGGAGAAGGATGC-3'

Other clones with same target sequence:

(none)