Construct: shRNA TRCN0000121280

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005157.3-1287s1c1
DNA Barcode:
GCTTTGGGAAATTGCTACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABL1 (25)
Gene Description:
ABL proto-oncogene 1, non-receptor tyrosine kinase
Transcript:
RefSeq NM_005157.3 (NON-CURRENT)
Match location:
Position 1287 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121280)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGGAAATTGCTACCTA
Hairpin Sequence:
5'-CCGG-GCTTTGGGAAATTGCTACCTA-CTCGAG-TAGGTAGCAATTTCCCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGGAAATTGCTACCTACTCGAGTAGGTAGCAATTTCCCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGGAAATTGCTACCTACTCGAGTAGGTAGCAATTTCCCAAAGC-3'

Other clones with same target sequence:

(none)