Construct: shRNA TRCN0000121299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000875.2-3879s1c1
DNA Barcode:
CTTCTACTACAGCGAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGF1R (3480)
Gene Description:
insulin like growth factor 1 receptor
Transcript:
RefSeq NM_000875.2 (NON-CURRENT)
Match location:
Position 3879 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTACTACAGCGAGGAGAA
Hairpin Sequence:
5'-CCGG-CTTCTACTACAGCGAGGAGAA-CTCGAG-TTCTCCTCGCTGTAGTAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTACTACAGCGAGGAGAACTCGAGTTCTCCTCGCTGTAGTAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTACTACAGCGAGGAGAACTCGAGTTCTCCTCGCTGTAGTAGAAG-3'

Other clones with same target sequence:

(none)