Construct: shRNA TRCN0000121318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005607.3-2779s1c1
DNA Barcode:
CCGATTGGAAACCAACATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Gene Description:
protein tyrosine kinase 2
Transcript:
RefSeq NM_005607.3 (NON-CURRENT)
Match location:
Position 2779 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATTGGAAACCAACATATA
Hairpin Sequence:
5'-CCGG-CCGATTGGAAACCAACATATA-CTCGAG-TATATGTTGGTTTCCAATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATTGGAAACCAACATATACTCGAGTATATGTTGGTTTCCAATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATTGGAAACCAACATATACTCGAGTATATGTTGGTTTCCAATCGG-3'

Other clones with same target sequence:

  1. TRCN0000332999