Construct: shRNA TRCN0000121344
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025605.1-185s1c1
- DNA Barcode:
- CCAACTACCATAGCCTACCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Lamtor1 (66508)
- Gene Description:
- late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
- Transcript:
- RefSeq NM_025605.1 (NON-CURRENT)
- Match location:
- Position 185 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000121344)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAACTACCATAGCCTACCTT
- Hairpin Sequence:
-
5'-CCGG-CCAACTACCATAGCCTACCTT-CTCGAG-AAGGTAGGCTATGGTAGTTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAACTACCATAGCCTACCTTCTCGAGAAGGTAGGCTATGGTAGTTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAACTACCATAGCCTACCTTCTCGAGAAGGTAGGCTATGGTAGTTGG-3'
Other clones with same target sequence:
(none)
