Construct: shRNA TRCN0000121347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027206.1-875s1c1
DNA Barcode:
GCTGACTGAAGAAGAAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfaip8l2 (69769)
Gene Description:
tumor necrosis factor, alpha-induced protein 8-like 2
Transcript:
RefSeq NM_027206.1 (NON-CURRENT)
Match location:
Position 875 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTGAAGAAGAAGTGAA
Hairpin Sequence:
5'-CCGG-GCTGACTGAAGAAGAAGTGAA-CTCGAG-TTCACTTCTTCTTCAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTGAAGAAGAAGTGAACTCGAGTTCACTTCTTCTTCAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTGAAGAAGAAGTGAACTCGAGTTCACTTCTTCTTCAGTCAGC-3'

Other clones with same target sequence:

(none)