Construct: shRNA TRCN0000121361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145448.2-1229s1c1
DNA Barcode:
ACCACTGGATTCCCAACACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9030617O03Rik (217830)
Gene Description:
RIKEN cDNA 9030617O03 gene
Transcript:
RefSeq NM_145448.2 (NON-CURRENT)
Match location:
Position 1229 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACTGGATTCCCAACACAT
Hairpin Sequence:
5'-CCGG-ACCACTGGATTCCCAACACAT-CTCGAG-ATGTGTTGGGAATCCAGTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACTGGATTCCCAACACATCTCGAGATGTGTTGGGAATCCAGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACTGGATTCCCAACACATCTCGAGATGTGTTGGGAATCCAGTGGT-3'

Other clones with same target sequence:

(none)