Construct: shRNA TRCN0000121363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031397.1-419s1c1
DNA Barcode:
CGCGTCACATTGAAGATGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bicc1 (83675)
Gene Description:
BicC family RNA binding protein 1
Transcript:
RefSeq NM_031397.1 (NON-CURRENT)
Match location:
Position 419 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGTCACATTGAAGATGGAT
Hairpin Sequence:
5'-CCGG-CGCGTCACATTGAAGATGGAT-CTCGAG-ATCCATCTTCAATGTGACGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTCACATTGAAGATGGATCTCGAGATCCATCTTCAATGTGACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTCACATTGAAGATGGATCTCGAGATCCATCTTCAATGTGACGCG-3'

Other clones with same target sequence:

  1. TRCN0000314291