Construct: shRNA TRCN0000121372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175160.2-1205s1c1
DNA Barcode:
CCTGCTCTGCTTCCACATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc1 (70796)
Gene Description:
zinc finger, DHHC domain containing 1
Transcript:
RefSeq NM_175160.2 (NON-CURRENT)
Match location:
Position 1205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCTGCTTCCACATTTA
Hairpin Sequence:
5'-CCGG-CCTGCTCTGCTTCCACATTTA-CTCGAG-TAAATGTGGAAGCAGAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCTGCTTCCACATTTACTCGAGTAAATGTGGAAGCAGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCTGCTTCCACATTTACTCGAGTAAATGTGGAAGCAGAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000130583