Construct: shRNA TRCN0000121405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024413.1-825s1c1
DNA Barcode:
CAGGTGGAGTTCTATGCTTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekhf1 (72287)
Gene Description:
pleckstrin homology domain containing, family F (with FYVE domain) member 1
Transcript:
RefSeq NM_024413.1 (NON-CURRENT)
Match location:
Position 825 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGTGGAGTTCTATGCTTCT
Hairpin Sequence:
5'-CCGG-CAGGTGGAGTTCTATGCTTCT-CTCGAG-AGAAGCATAGAACTCCACCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGTGGAGTTCTATGCTTCTCTCGAGAGAAGCATAGAACTCCACCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGTGGAGTTCTATGCTTCTCTCGAGAGAAGCATAGAACTCCACCTG-3'

Other clones with same target sequence:

  1. TRCN0000340987