Construct: shRNA TRCN0000121410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198249.1-3313s1c1
DNA Barcode:
GCGTCTAGTGTCTGAACTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef40 (268739)
Gene Description:
Rho guanine nucleotide exchange factor (GEF) 40
Transcript:
RefSeq NM_198249.1 (NON-CURRENT)
Match location:
Position 3313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTAGTGTCTGAACTGAT
Hairpin Sequence:
5'-CCGG-GCGTCTAGTGTCTGAACTGAT-CTCGAG-ATCAGTTCAGACACTAGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTAGTGTCTGAACTGATCTCGAGATCAGTTCAGACACTAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTAGTGTCTGAACTGATCTCGAGATCAGTTCAGACACTAGACGC-3'

Other clones with same target sequence:

(none)