Construct: shRNA TRCN0000121420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181413.2-1070s1c1
DNA Barcode:
CGCCCTGATTGAAGATCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anks1 (224650)
Gene Description:
ankyrin repeat and SAM domain containing 1
Transcript:
RefSeq NM_181413.2 (NON-CURRENT)
Match location:
Position 1070 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCCTGATTGAAGATCACAT
Hairpin Sequence:
5'-CCGG-CGCCCTGATTGAAGATCACAT-CTCGAG-ATGTGATCTTCAATCAGGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCCTGATTGAAGATCACATCTCGAGATGTGATCTTCAATCAGGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCCTGATTGAAGATCACATCTCGAGATGTGATCTTCAATCAGGGCG-3'

Other clones with same target sequence:

(none)