Construct: shRNA TRCN0000121443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175543.2-1729s1c1
DNA Barcode:
CGGGATCAGAATGATGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab11fip4 (268451)
Gene Description:
RAB11 family interacting protein 4 (class II)
Transcript:
RefSeq NM_175543.2 (NON-CURRENT)
Match location:
Position 1729 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATCAGAATGATGATTTA
Hairpin Sequence:
5'-CCGG-CGGGATCAGAATGATGATTTA-CTCGAG-TAAATCATCATTCTGATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATCAGAATGATGATTTACTCGAGTAAATCATCATTCTGATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATCAGAATGATGATTTACTCGAGTAAATCATCATTCTGATCCCG-3'

Other clones with same target sequence:

(none)