Construct: shRNA TRCN0000121578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015202.1-160s1c1
DNA Barcode:
CCGGATATTAAAGCATTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA0556 (23247)
Gene Description:
KIAA0556
Transcript:
RefSeq NM_015202.1 (NON-CURRENT)
Match location:
Position 160 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATATTAAAGCATTTGAA
Hairpin Sequence:
5'-CCGG-CCGGATATTAAAGCATTTGAA-CTCGAG-TTCAAATGCTTTAATATCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATATTAAAGCATTTGAACTCGAGTTCAAATGCTTTAATATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATATTAAAGCATTTGAACTCGAGTTCAAATGCTTTAATATCCGG-3'

Other clones with same target sequence:

(none)