Construct: shRNA TRCN0000121618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024294.2-1732s1c1
DNA Barcode:
GCCAATGAGTTATCACCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ILRUN (64771)
Gene Description:
inflammation and lipid regulator with UBA-like and NBR1-like domains
Transcript:
RefSeq NM_024294.2 (NON-CURRENT)
Match location:
Position 1732 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGAGTTATCACCCTTT
Hairpin Sequence:
5'-CCGG-GCCAATGAGTTATCACCCTTT-CTCGAG-AAAGGGTGATAACTCATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGAGTTATCACCCTTTCTCGAGAAAGGGTGATAACTCATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGAGTTATCACCCTTTCTCGAGAAAGGGTGATAACTCATTGGC-3'

Other clones with same target sequence:

(none)