Construct: shRNA TRCN0000121638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015186.1-9157s1c1
DNA Barcode:
GCTTAGTTTCTGGATTTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS13A (23230)
Gene Description:
vacuolar protein sorting 13 homolog A
Transcript:
RefSeq NM_015186.1 (NON-CURRENT)
Match location:
Position 9157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGTTTCTGGATTTGTTA
Hairpin Sequence:
5'-CCGG-GCTTAGTTTCTGGATTTGTTA-CTCGAG-TAACAAATCCAGAAACTAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGTTTCTGGATTTGTTACTCGAGTAACAAATCCAGAAACTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGTTTCTGGATTTGTTACTCGAGTAACAAATCCAGAAACTAAGC-3'

Other clones with same target sequence:

(none)