Construct: shRNA TRCN0000121799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173551.1-740s1c1
DNA Barcode:
CTGACTGGAATCCTTAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKS6 (203286)
Gene Description:
ankyrin repeat and sterile alpha motif domain containing 6
Transcript:
RefSeq NM_173551.1 (NON-CURRENT)
Match location:
Position 740 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACTGGAATCCTTAAGAAA
Hairpin Sequence:
5'-CCGG-CTGACTGGAATCCTTAAGAAA-CTCGAG-TTTCTTAAGGATTCCAGTCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACTGGAATCCTTAAGAAACTCGAGTTTCTTAAGGATTCCAGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACTGGAATCCTTAAGAAACTCGAGTTTCTTAAGGATTCCAGTCAG-3'

Other clones with same target sequence:

(none)