Construct: shRNA TRCN0000121801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376428.1-2716s1c1
DNA Barcode:
CGCTTGATTATCTCAATAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLJ44796 (401209)
Gene Description:
uncharacterized FLJ44796
Transcript:
RefSeq XM_376428.1 (NON-CURRENT)
Match location:
Position 2716 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTGATTATCTCAATAGAT
Hairpin Sequence:
5'-CCGG-CGCTTGATTATCTCAATAGAT-CTCGAG-ATCTATTGAGATAATCAAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTGATTATCTCAATAGATCTCGAGATCTATTGAGATAATCAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTGATTATCTCAATAGATCTCGAGATCTATTGAGATAATCAAGCG-3'

Other clones with same target sequence:

(none)