Construct: shRNA TRCN0000121872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153488.3-1220s1c1
DNA Barcode:
GCAGTGAGTTTCTGTTCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA2B (266740)
Gene Description:
MAGE family member A2B
Transcript:
RefSeq NM_153488.3 (NON-CURRENT)
Match location:
Position 1220 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGAGTTTCTGTTCTGTT
Hairpin Sequence:
5'-CCGG-GCAGTGAGTTTCTGTTCTGTT-CTCGAG-AACAGAACAGAAACTCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGAGTTTCTGTTCTGTTCTCGAGAACAGAACAGAAACTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGAGTTTCTGTTCTGTTCTCGAGAACAGAACAGAAACTCACTGC-3'

Other clones with same target sequence:

(none)