Construct: shRNA TRCN0000121929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007525.1-5714s1c1
DNA Barcode:
CCCAGGGAAATGAAACCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NWD1 (284434)
Gene Description:
NACHT and WD repeat domain containing 1
Transcript:
RefSeq NM_001007525.1 (NON-CURRENT)
Match location:
Position 5714 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGGAAATGAAACCAAAT
Hairpin Sequence:
5'-CCGG-CCCAGGGAAATGAAACCAAAT-CTCGAG-ATTTGGTTTCATTTCCCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGGAAATGAAACCAAATCTCGAGATTTGGTTTCATTTCCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGGAAATGAAACCAAATCTCGAGATTTGGTTTCATTTCCCTGGG-3'

Other clones with same target sequence:

(none)