Construct: shRNA TRCN0000122074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173545.1-516s1c1
DNA Barcode:
CCCGTGATTAATTTACCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APLF (200558)
Gene Description:
aprataxin and PNKP like factor
Transcript:
RefSeq NM_173545.1 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTGATTAATTTACCTCAT
Hairpin Sequence:
5'-CCGG-CCCGTGATTAATTTACCTCAT-CTCGAG-ATGAGGTAAATTAATCACGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTGATTAATTTACCTCATCTCGAGATGAGGTAAATTAATCACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTGATTAATTTACCTCATCTCGAGATGAGGTAAATTAATCACGGG-3'

Other clones with same target sequence:

(none)