Construct: shRNA TRCN0000122136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016045.1-1520s1c1
DNA Barcode:
GCATGATATGTCAGGTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRELID3B (51012)
Gene Description:
PRELI domain containing 3B
Transcript:
RefSeq NM_016045.1 (NON-CURRENT)
Match location:
Position 1520 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGATATGTCAGGTTATTT
Hairpin Sequence:
5'-CCGG-GCATGATATGTCAGGTTATTT-CTCGAG-AAATAACCTGACATATCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGATATGTCAGGTTATTTCTCGAGAAATAACCTGACATATCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGATATGTCAGGTTATTTCTCGAGAAATAACCTGACATATCATGC-3'

Other clones with same target sequence:

(none)