Construct: shRNA TRCN0000122139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017657.2-1335s1c1
DNA Barcode:
GCTGATAATTCAGAAGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFTPH (54812)
Gene Description:
aftiphilin
Transcript:
RefSeq NM_017657.2 (NON-CURRENT)
Match location:
Position 1335 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATAATTCAGAAGCCATT
Hairpin Sequence:
5'-CCGG-GCTGATAATTCAGAAGCCATT-CTCGAG-AATGGCTTCTGAATTATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATAATTCAGAAGCCATTCTCGAGAATGGCTTCTGAATTATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATAATTCAGAAGCCATTCTCGAGAATGGCTTCTGAATTATCAGC-3'

Other clones with same target sequence:

(none)