Construct: shRNA TRCN0000122167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006198.1-119s1c1
DNA Barcode:
GATGGACAGAAGAAAGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCP4 (5121)
Gene Description:
Purkinje cell protein 4
Transcript:
RefSeq NM_006198.1 (NON-CURRENT)
Match location:
Position 119 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGACAGAAGAAAGTTCAA
Hairpin Sequence:
5'-CCGG-GATGGACAGAAGAAAGTTCAA-CTCGAG-TTGAACTTTCTTCTGTCCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGACAGAAGAAAGTTCAACTCGAGTTGAACTTTCTTCTGTCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGACAGAAGAAAGTTCAACTCGAGTTGAACTTTCTTCTGTCCATC-3'

Other clones with same target sequence:

(none)