Construct: shRNA TRCN0000122181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144713.1-393s1c1
DNA Barcode:
GCAACAAAGTAGAGCTAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RMDN2 (151393)
Gene Description:
regulator of microtubule dynamics 2
Transcript:
RefSeq NM_144713.1 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAAAGTAGAGCTAACTT
Hairpin Sequence:
5'-CCGG-GCAACAAAGTAGAGCTAACTT-CTCGAG-AAGTTAGCTCTACTTTGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAAAGTAGAGCTAACTTCTCGAGAAGTTAGCTCTACTTTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAAAGTAGAGCTAACTTCTCGAGAAGTTAGCTCTACTTTGTTGC-3'

Other clones with same target sequence:

(none)