Construct: shRNA TRCN0000122343

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138414.1-962s1c1
DNA Barcode:
CCTGTTTGAAGACACCTCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGF29 (112869)
Gene Description:
SAGA complex associated factor 29
Transcript:
RefSeq NM_138414.1 (NON-CURRENT)
Match location:
Position 962 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122343)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTTGAAGACACCTCCTA
Hairpin Sequence:
5'-CCGG-CCTGTTTGAAGACACCTCCTA-CTCGAG-TAGGAGGTGTCTTCAAACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTTGAAGACACCTCCTACTCGAGTAGGAGGTGTCTTCAAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTTGAAGACACCTCCTACTCGAGTAGGAGGTGTCTTCAAACAGG-3'

Other clones with same target sequence:

(none)