Construct: shRNA TRCN0000122351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007089.1-417s1c1
DNA Barcode:
CAACCCTGTCAAGATTACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RESP18 (389075)
Gene Description:
regulated endocrine specific protein 18
Transcript:
RefSeq NM_001007089.1 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCCTGTCAAGATTACCTA
Hairpin Sequence:
5'-CCGG-CAACCCTGTCAAGATTACCTA-CTCGAG-TAGGTAATCTTGACAGGGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCCTGTCAAGATTACCTACTCGAGTAGGTAATCTTGACAGGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCCTGTCAAGATTACCTACTCGAGTAGGTAATCTTGACAGGGTTG-3'

Other clones with same target sequence:

(none)