Construct: shRNA TRCN0000122392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016145.1-266s1c1
DNA Barcode:
GCCGAACAAAGTGCTGAGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR83OS (51398)
Gene Description:
WD repeat domain 83 opposite strand
Transcript:
RefSeq NM_016145.1 (NON-CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAACAAAGTGCTGAGGTA
Hairpin Sequence:
5'-CCGG-GCCGAACAAAGTGCTGAGGTA-CTCGAG-TACCTCAGCACTTTGTTCGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAACAAAGTGCTGAGGTACTCGAGTACCTCAGCACTTTGTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAACAAAGTGCTGAGGTACTCGAGTACCTCAGCACTTTGTTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000319117